Gene description for Hspa1b
Gene name heat shock protein 1B
Gene symbol Hspa1b
Other names/aliases HSP70B1
Hsp70
Hsp70-1
Hsp70.1
hsp68
Species Mus musculus
 Experiment description of studies that identified Hspa1b in Lung cancer cells

1
Experiment ID2890
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 28928431   
OrganismMus musculus
Experiment descriptionTumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90
AuthorsZhang G, Liu Z, Ding H, Zhou Y, Doan HA, Sin KWT, Zhu ZJ, Flores R, Wen Y, Gong X, Liu Q, Li YP.
Journal name Nat Commun
Publication year2017
SampleLung cancer cells
Sample nameLLC
Isolation/purification methodsCentrifugal ultrafiltration
ExoQuick Kit
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

2
Experiment ID2891
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 28928431   
OrganismMus musculus
Experiment descriptionTumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90
AuthorsZhang G, Liu Z, Ding H, Zhou Y, Doan HA, Sin KWT, Zhu ZJ, Flores R, Wen Y, Gong X, Liu Q, Li YP.
Journal name Nat Commun
Publication year2017
SampleLung cancer cells
Sample nameLLC - immunoprecipitation IgG control supernatant
Isolation/purification methodsCentrifugal ultrafiltration
ExoQuick Kit
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

3
Experiment ID2892
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 28928431   
OrganismMus musculus
Experiment descriptionTumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90
AuthorsZhang G, Liu Z, Ding H, Zhou Y, Doan HA, Sin KWT, Zhu ZJ, Flores R, Wen Y, Gong X, Liu Q, Li YP.
Journal name Nat Commun
Publication year2017
SampleLung cancer cells
Sample nameLLC - immunoprecipitation of CD9 precipitated pellet
Isolation/purification methodsCentrifugal ultrafiltration
ExoQuick Kit
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID3372
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 33510128   
OrganismMus musculus
Experiment descriptionExtracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer cachexia
AuthorsHu W, Xiong H, Ru Z, Zhao Y, Zhou Y, Xie K, Xiao W, Xiong Z, Wang C, Yuan C, Shi J, Du Q, Zhang X, Yang H.
Journal name Cell Death Dis
Publication year2021
SampleLung cancer cells
Sample nameLLC
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID3377
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 36581870   
OrganismMus musculus
Experiment descriptionExosomal IL-8 derived from Lung Cancer and Colon Cancer cells induced adipocyte atrophy via NF-κB signaling pathway
AuthorsXiong H, Ye J, Xie K, Hu W, Xu N, Yang H.
Journal name Lipids Health Dis
Publication year2022
SampleLung cancer cells
Sample nameLLC
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Qiagen Exosome Purification Kit
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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